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The reaction consisted of a hot-start protocol with an initial reverse transcription at 50C for 30 min, a PCR activation step at 95C for 15 min, 35 cycles at 95C for 30 s, 55C for 30 s, and 72C for 1 min, and a final elongation at 72C for 7 min

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Those on tirzepatide also lose 5% more total body weight compared to those on semaglutide

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